Pregnancy Test Results: What One Line Means - Obstetrics and Gynecology

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How to Use a Pregnancy Test Stick


Hello doctor, I tested for pregnancy using a pregnancy test stick on the 11th and 13th mornings after intercourse, and both times it only showed one line.
However, my girlfriend's period is now three days late.
I would like to know if these pregnancy test results are accurate.

abc, 20~29 year old female. Ask Date: 2021/06/02

Dr. Huang Yiwen reply Obstetrics and Gynecology


Hello Mr.
ABC: It is recommended that you wait until your girlfriend's menstrual period is overdue before taking a pregnancy test for more accurate results.

Reply Date: 2021/06/02

More Info


When interpreting pregnancy test results, it's essential to understand how these tests work and the timing of testing in relation to ovulation and potential implantation. A pregnancy test typically detects the presence of human chorionic gonadotropin (hCG), a hormone produced shortly after a fertilized egg attaches to the uterine lining.
In your case, you mentioned that you conducted pregnancy tests on the 11th and 13th days after sexual intercourse, and both tests showed only one line. This result generally indicates that there is no detectable hCG in your girlfriend's urine at the time of testing, suggesting that she is not pregnant. However, there are several factors to consider that could influence the accuracy of these results.

1. Timing of the Test: The timing of the test is crucial. If ovulation occurred later than expected, it could take longer for hCG levels to rise to a detectable level. Typically, hCG can be detected in urine about 14 days after ovulation. If your girlfriend's menstrual cycle is irregular or if she ovulated later than usual, it might be too early to test accurately.

2. Sensitivity of the Test: Different pregnancy tests have varying sensitivities to hCG. Some tests can detect lower levels of hCG than others. If you used a test with lower sensitivity, it might not pick up a pregnancy even if one exists. It might be worth trying a different brand or a more sensitive test.

3. Testing Method: The accuracy of the test can also depend on how it was used. Ensure that the test was used according to the manufacturer's instructions, including the timing of reading the results. Reading the test too early or too late can lead to misinterpretation.

4. Menstrual Cycle Considerations: You mentioned that your girlfriend's period is three days late. A missed period can be a sign of pregnancy, but it can also be caused by other factors such as stress, hormonal imbalances, or changes in weight. If her period does not arrive soon, it would be advisable to wait a few days and then retest, preferably using the first morning urine, which typically has the highest concentration of hCG.

5. Follow-Up: If the tests continue to show one line and her period does not start, it would be wise for her to consult a healthcare provider. A blood test can provide a more definitive answer regarding pregnancy, as it can detect lower levels of hCG than urine tests.

In summary, while two tests showing one line typically suggest that your girlfriend is not pregnant, the timing of the tests, the sensitivity of the test used, and her menstrual cycle should all be taken into account. If her period does not arrive soon, consider retesting in a few days or consulting a healthcare professional for further evaluation.

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